Project 2: Synthesis of new Fluorescence probes will be used for

Project 2: Synthesis of new Fluorescence probes will be used for determination of
nucleic acids and Investigation of Interactions with spectoflourometric method.
Quantitative analysis of nucleic acids, molecular biology, biotechnology and medical
diagnostics is very important in many areas especially. High chemical stability compared with
DNA and other biological compounds, for the determination of nucleic acid-fast, accurate and
easily allows for the development of analytical methods. In the literature for the
spectrophotometric determination of nucleic acids, such as chemiluminescence, light
scattering, electrochemistry and chromatography methods have been proposed. However, in
recent years, great interest shown in fluorometric methods. Nucleic acids have a very low
intensity and spectral characteristics of fluorescence emission is very difficult to direct
examination. Indirectly in fluorometric method, fluorescence inserts, for example, fluorescent
dyes, metal ions and metal complexes are used. the interaction of fluorescence insert with the
nucleic acid is carried out through three main connecting model, these are intercalation,
groove binding and electrostatic interactions. When fluorescence inserts connected with
biomolecule which has slighly or non fluorescence properties, they gain fluorescence
properties or thier spectral properits changed and this allows to monitor biomolecule structure
and function. The inserts interact with many biomolecules such as proteins, nucleic acids,
hormones, growth factors that allow us to examine the structure and function of these species.
In this context, several fluorescence-based inserts coumarin synthesized by our group. In this
field, new fluorescence compounds are obtained easily and had low toxic effect will be
targeted to synthesize.
General Structure of the synthesized compounds;
1. Fluorescence-based Fenantridin probes ;
_
Br + N
R
N
N
H
H
R
R:Benzoil, Acetyl, Benzoxyacetyl, Etoxyacetyl, Metoxyacetyl,
Propoxyacetyl, Butoxyacetyl, phenoxyacetyl etc.
2. Fluorescence-based acridine probes
R
N
NH
O C H3
R:
NH
OC 2 H5
,
O
O
O
O
R
OC 3 H 8
O
O
,
O
,
O
Completed project : Nükleik Asitlerin Tayininde Kullanılabilecek Yeni Floresant Uçların
Sentezlenmesi ve Etkileşimlerin Spektroflorimetrik Yöntemle İncelenmesi TÜBİTAK
Projesi, Proje No: TBAG-107T391, 2007-2009.
Selective Publications:
1. Akbay, N., Seferoğlu, Z. ve Gökoğlu, E. “Fluorescence interaction and determination
of calf thymus DNA with two ethidium derivatives”, Journal of Fluorescence, 19(6),
1045-1054 (2009).
2. Akbay, N., Seferoğlu, Z. ve Gökoğlu, E. “Interactions of Two Ethidium Derivatives
with Calf Thymus DNA and Serum Albumins Using Fluorescence Quenching Method”,
Current Analytical Chemistry, 6(4),334-340 (2010).
3. Göker F., Akbay, N., Seferoğlu, Z. ve Gökoğlu, E. “Interactions of Acridine
Derivatives with Bouvine Serum Albumin: A Fluorescence Quenching”, 5nd Black Sea
Basın Conference on Analytical Chemistry, 182, Ordu-Fatsa/Turkey, 23-26 September
2009 (En iyi Poster Ödülü).