Biocem Sağlık Ürünleri San. ve Tic. Ltd. Şti.
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Biocem Sağlık Ürünleri San. ve Tic. Ltd. Şti. Hakkımızda BioCem Ltd. Şti. Türkiye’de in-vitro diagnostik, araştırma ve tıbbi malzeme alanlarında hizmet veren distribütör bir kuruluştur. Şirketimiz kusursuz hizmet anlayışı ve kendi alanında üstünlüğü kanıtlanmış olan tedarikçileriyle sağlam temellere dayanan bir işbirliği içerisinde hizmet vermektedir. Misyonumuz, değerli müşterilerimize sağlık ve hayat bilimi sektörünü destekleme hedefinde kusursuz kalite ve memnun edici servisi sunmaktır. BioCem her biri alanında uzmanlaşmış kadrosuyla müşterileriyle yakın ilişki içerisinde kaliteyi sunmaktadır. Kurulduğu günden beri, araştırma çalışmalarına verdiği destek ve yatırım, müşteri memnuniyetinin sağlanması ve zamanında teslimat ile sağlık pazarında saygın bir yer edinmiştir. Genişleyen pazar payımızı artırabilmek adına, kararlı ve istikrarlı adımlarla çalışmaya devam etmekteyiz. Saygın tedarikçi firmalarımızdan değerli müşterilerimize kadar olan bir zincir anlayışı içerisinde, gelecekteki sayısız ortak başarıda birlikte çalışmak dileğiyle… Temsilciliklerimiz www.biocem.com.tr 1 Affymetrix Inc. ExoSAP-IT USB® ExoSAP-IT® PCR Product Cleanup means the ease of one-step purification. The ExoSAP-IT method is a unique, one-tube, one-step, enzymatic cleanup reagent for PCR product cleanup. • 100% sample recovery – no loss of PCR products regardless of the fragment size • Removes excess primers and dNTPs – does not interfere with downstream applications • Superior results with ExoSAP-IT reagent – improve accuracy with higher yields and full PCR product recovery • Cited in over 7,000 publications – the enzymatic PCR cleanup solution used by leading scientists USB ExoSAP-IT PCR Product Cleanup – available for standard and high-throughput, automated platforms ExoSAP-IT PCR Product Cleanup 78250 20 Reactions 78200 100 Reactions 78201 500 Reactions 78202 2,000 Reactions 78205 5,000 Reactions PCR products cleaned up with ExoSAP-­‐IT reagent prior to automated sequencing display superior sequencing results compared to the un-­‐cleaned equivalent www.biocem.com.tr 2 Affymetrix Inc. Biochemicals USB biochemicals provide reliable, repeatable results. You demand dependable products, and we deliver the highest-purity biochemicals and reagents with the proper documentation, every time. Top life science companies rely on USB biochemicals to consistently meet their manufacturing requirements. Acrylamides Detergents ACS Grade Biochemicals Enzymes Agaroses General/Ultrapure Biochemicals Albumins Proteins Antibiotics Substrates Bacterial Culture Media Sugars Buffers Vitamins Master Mixes & Kits Markers DNA Polymerases www.biocem.com.tr 3 Affymetrix Inc. VeriQuest VeriQuest Fast SYBR Green qPCR Master Mix (2X) A 2X pre-mixed formulation of SYBR Green qPCR Master Mix designed for fast mode cycling conditions Part # Description Unit Size 75690 100 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 1 ml for 100 reactions (20 µl) 75690 100 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 5 ml for 500 reactions (20 µl) 75690 1000 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 2 x 5 ml for 1,000 reactions (20 µl) 75690 2500 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 5 x 5 ml for 2,500 reactions (20 µl) 75690 5000 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 5 x 5 ml for 2,500 reactions (20 µl) VeriQuest SYBR Green qPCR Master Mix (2X) Part # Description Unit Size 75600 40 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 1 ml for 40 reactions (50 µl) 75600 200 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 5 ml for 200 reactions (50 µl) 75600 400 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 2 x 5 ml for 400 reactions (50 µl) 75600 1000 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 5 x 5 ml for 1,000 reactions (50 µl) 75600 2000 RXN VeriQuest Fast SYBR Green qPCR Master Mix (2X) 10 x 5 ml for 2,000 reactions (50 µl) www.biocem.com.tr 4 Affymetrix Inc. How to select the correct VeriQuest MM www.biocem.com.tr 5 ARRAYS C-Series L-Series Quantibody G-Series E-Series Detection Chemilluminescent Solid Support Membrane Desingprinciple Sandwich ELISA Results Semi-quantitative Detection Fluorescent or Chemilluminescent Solid Support Glass Slide or Membrane Desingprinciple Direct Labelling (Biotin) Results Semi-quantitative Detection Fluorescent Solid Support Glass Slide Desingprinciple Sandwich ELISA Results Quantitative Detection Fluorescent Solid Support Glass Slide Desingprinciple Sandwich ELISA Results Semi-quantitative Detection Chemilluminescent Solid Support Membrane Desingprinciple Competitive ELISA Results Semi-quantitative or Quantitative Working sample volume: 1,000 µl (1 ml) Advantages & Features: No specialized equipment required Easy to use High specificity Low background, high sample diversity, etc. Working sample volume: 20-100 µl Advantages & Features; Hundreds of molecules can be screened simultaneously, Lowest cost per target High specificity Compatible with serum, plasma, and cell culture media Working sample volume: 50-100 µl Advantages & Features: Lower sample consumption Easy to use; high throughput Wider dynamic range (up to 5 orders of magnitude) Low background, high sample diversity, etc. Working sample volume: 50-100 µl Advantages & Features: Lower sample consumption Easy to use; high throughput Wider dynamic range (up to 5 orders of magnitude) Low background, high sample diversity, etc. Working sample volume: inquire Advantages & Features: No specialized equipment required Easy to use High specificity Low background, high sample diversity, etc. CUSTUMIZE YOUR ARRAY www.biocem.com.tr 6 ARRAYS C-SERİES CUSTUMIZE YOUR ARRAY www.biocem.com.tr 7 ARRAYS QUANTİBODY: MULTİPLEX ELISA ARRAY PROTEINS Custom Recombinant Protein Service Globulins Adhesion Molecule/Cell Surface Heat Shock Proteins Adipokine/Obesity-related Hormones and Steroids Autoimmune/Allergy Immunoglobulins Bacterial/Fungal/Parasitic/Viral Antigens Lipoproteins Blood Coagulation Proteins Miscellaneous Cancer-related Markers Molecular Biology Cardiac Markers Neuroscience Collagen & Extracellular Matrix Proteins Phosphorylation/Apoptosis/Signal Transduction/Regulation Cytokines/Chemokines/Growth Factors/Receptors Proteases & Other Enzymes Cytoskeleton Protein Kinases Drug Derivative Proteins CUSTUMIZE YOUR PROTEIN www.biocem.com.tr 8 ELISA KITS 1 Plate Kit / 2 Plate Kit / 5 Plate Kit 1 Plate Kit / 2 Plate Kit / 5 Plate Kit 1 Plate Kit / 2 Plate Kit / 5 Plate Kit 1 Plate Kit / 2 Plate Kit / 5 Plate Kit www.biocem.com.tr 9 ANTIBODYS Adhesion molecule/Cell Surface Adipokine/Obesity-related Autoimmune/Allergy Bacterial/Fungal/Parasitic/Viral Antigens Blood Coagulation Proteins Calcium Channel Calcium signaling Cancer-related Markers Cardiac Markers Cell Cycle Collagen & Extracellular Matrix Proteins Cytokines/Chemokines/Growth Factors/Receptors Cytoskeleton Drug/Derivative Enzyme/Protease Globulins Glycoproteins Heat Shock Proteins Hormones and Steroids Immunodetection Kit Immunoglobulins Lipoproteins MHC Class Miscellaneous Neuroscience NF-kB signaling pathway Phosphorylation/Apoptosis/Signal Transduction/Regulation Platelet and Homeostasis Proteins Proteases & Other Enzymes Protein Kinases Stem Cell Markers T-Cell Receptors Tag Transcription and its regulation Transmembrane Protein and Receptor www.biocem.com.tr 10 Hycult Biotech ELISA & ANTIBODY ELISA Specific ELISA for Innate Immunity, Cell & Tissue damage, Inflammation, Disease-related Products 1x 96 det. and 2 x 96 det. ELISA Kits Standard protocol of 3.5 hour PRODUCT MPO ELISA KIT (h,m,r) 1x96 det. / 2 x 96 det. NGAL ELISA KIT (h) 1x96 det./ 2 x 96 det. Calprotectin ELISA KIT (h) 1x96 det. / 2 x 96 det. Pentraxin 3 ELISA KIT (h) 1x96 det. / 2 x 96 det. Lactoferrin ELISA KIT (h) 1x96 det. / 2 x 96 det. Nitrotyrosine ELISA KIT (h) 1x96 det. / 2 x 96 det. H-FABP ELISA KIT (h,m,r) 1x96 det. / 2 x 96 det. L-FABP ELISA KIT (h) 1x96 det. / 2 x 96 det. I-FABP ELISA KIT (h) 1x96 det. / 2 x 96 det. LAL Chromogenic Endpoint ASSAY 3 x 96 det ANTI BODY Specific primary and secondary antibodies for FC,FS,F P,FI,IP,IA,W The antibodies are arranged by species: human, mouse and rat. PRODUCT CD14,Human,mAb 18D11 CD36 Human, mAb FA6-152 DAF, Human, mAb D17 Beta-Catenin, Human, mAb 9F2 Pentraxin 3, Human, mAb, MNB4 TLR4, Human, mAb3C3 biotinylated JAM-A. Mouse, mAb, BV12 TLR4, Mouse, mAb Kat5-18 CD8,Rat, mAb 53-5D2 ZO-1,Human,pAb www.biocem.com.tr 11 Primary Antibodys BT-Laboratory Elisa Kits, • Over 7400 different Elisa Kits • For Human, Mouse, Rat, Rabbit, Bovine, Sheep, Cat, Horse, Chicken, Monkey, Fish, Goat, Canine • Validated for Serum, blood plasma,Saliva, Urine sample types. BT-Laboratory ELISA KITS Human Tumor necrosis factor α,TNF-α ELISA KIT Human Tumor necrosis factor α,TNF-α ELISA KIT Human Interferon α,IFN-α ELISA Kit Human Interleukin 1α,IL-1α ELISA Kit Canine Interferon γ ,IFN-γ ELISA Kit Canine Tissue factor,IF ELISA Kit Bovine Anti-Thrombin Ⅲ,AT-Ⅲ ELISA Kit Bovine malondialchehyche,MDA ELISA Kit Bovine Endothelin 1,ET-1 ELISA Kit Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone,MIS/AMH ELISA Kit Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit Mouse bradykinin,BK ELISA Kit Mouse Insulin Like Growth Factor Binding Protein 3 (IGFBP3)ELISA KIT Chicken Interleukin 1α,IL-1α ELISA Kit Human myeloperoxidase,MPO ELISA Kit Human Calf intestinal alkaline phosphatase,CIAP ELISA Kit Human myeloperoxidase,MPO ELISA Kit Human Thyroid-Peroxidase,TPO ELISA Kit Human Matrix metalloproteinase 10,MMP-10 ELISA kit Human Endothelial nitric oxide synthase,eNOS ELISA Kit Human Super Oxidase Dimutase,SOD ELISA Kit Human nitric oxide synthase,NOS ELISA Kit Human Tumor necrosis factor soluble receptorⅡ,TNFsR-ⅡELISA KIT Human soluble tumor necrosis factor 1,sTNF-1 ELISA Kit Human reticulin IgG ELISA Kit Human reticulin IgA ELISA Kit Rat Interleukin 1,IL-1 ELISA Kit Rat Interleukin 1α ,IL-1α ELISA kit Rat Interleukin 1β,IL-1β ELISA Kit Rat osteonectin,ON ELISA Kit Rat Alpha-fetoprotein,AFP ELISA Kit Rat annexin Ⅴ,ANX-Ⅴ ELISA Kit Mouse eosinophil chemotactic factor,ECF ELISA Kit Mouse soluble endothelial protein C receptor,sEPCR ELISA Kit Mouse Interleukin 11,IL-11 ELISA KIT Mouse Matrix metalloproteinase 2/Gelatinase A(MMP-2/Gelatinase A)ELISA Kit Mouse Myoglobin,MYO/MB ELISA Kit www.biocem.com.tr 12 Intron Biotechnology Inc. PCR i-Taq™ DNA Polymerase (with Buffer, dNTPs) i-Taq™ PCR core Kit in high purity which is stable and effective for DNA amplification regardless the sort and reaction condition of template. INT25022…………….........…………..……….……….…….….500unit i-StarTaq™ DNA Polymerase (with Buffer, dNTPs) i-StarTaq™ PCR core kit having hot-start function for reduction on non-specific amplification and high specificity. INT25162..………………………..……….……….…….….500unit 2xPCR Master mix Solution 2x PCR Master mix Solution is designed to get the best result with simplicity that all components are premixed in a tube with 2x solution mix type. INT25027(i-Taq)….………………...................................0,5 ml x 2 vials INT25028(i-Taq)….……………………….........................1 ml x 5 vials INT25166(i-StarTaq)……….……….................................0,5 ml x 2 vials INT25186(i-pfu)….…………...…….…............….………..1 ml x 5 vials FastMix/Frenche PCR Premix Kit FastMix/FrencheTM PCR maximized sensitivity as well as amplification rate compared to thexisting Maxime series. By eliminating the artificial additive, all components are optimized for performing PCR and can be stable at room temperature up to months. INT25401.........………………………………………….......…96 Tubes INT25411…………………………………………………....…96 Tubes e-Myco™ Mycoplasma PCR Detection Mycoplasma are commonand serious contaminants of cell cultures. It has been shown that 30% to 87% of cell culturesare infected with mycoplasma. INT25235(detects 49 kind of mycoplasma).............................. 48 tubes INT25237 (detects 209 kind of mycoplasma)......…….…….…48 tubes www.biocem.com.tr 13 Intron Biotechnology Inc. PCR 6X Loading Dye Solution (XB) or(XO) 6X Loading Dye Solution(XB) is used to prepare DNA markers and samples for loading on agarose orpolyacrylamide gels. It is recommended for the routine DNA work use. Each Loading Dye contains two tracking dye, orange G dye and xylene cyanol FF for visual tracking of DNA migration during electrophoresis. INT21161 (XB) ............….…………………………………………. 5 ml INT21162 (XO) …..…………………………………………….……5 ml SiZer™-DNA Marker Solution INT24071 (20 ).....……………………….…………..……..……0,5 ml INT24072 (50 )….………………………..…………..……..……0,5 ml INT24073 (100 )........………………….……………..……...…....0,5 ml INT24074 (1000)......…………………..……………..….…..……0,5 ml INT24076(15k)….…………………………………….……..……0,5 ml RedSafe™ Nucleic Acid Staining Solution RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. INT21141.….…………………………………………………….0,5 ml Other M-MLV Reverse Transcriptase M-MLV Reverse Transcriptase is one of DNA polymerase and used for the synthesis of Single stranded RNA, DNA or complementary DNA strand from RNA:DNA hybrid like AMV RT. INT27032……………………………..……….……….……10.000 Unit www.biocem.com.tr 14 Intron Biotechnology Inc. Other AMV Reverse Transcriptase AMV Reverse Transcriptase is one of DNA polymerase and used for the synthesis of Single stranded RNA, DNA or complementary DNA strand from RNA:DNA hybrid. This is commonly used for cDNA synthesis from RNA. INT27021……………………………..…....…….…….…......…200 Unit Proteinase K Proteinase K is prified from Tritirachium album and non specific serine protease which has activity at pH, salt,surface active agent and temperature. INT32181……………………………..…....…….….......1ml(30 mg/ml) Ribonuclease A (RNase A) Ribonuclease A is endoribonuclease which is purified from bovine pancreas. This enzyme is useful to selectively remove RNA. INT27062……………………………..…....…….……0,5 ml(10 mg/ml) EtBr WiPER EtBr Wiper is an essential item for decontaminating Ethidium Bromide (EtBr) in the laboratory including the space, the surface of equipment, the surface of glassware, etc. INT21132……………………………..…....…….…….……2x200ml www.biocem.com.tr 15 Antigenix America Inc. Super-X Plex® Flow Cytometry Cytokine Assays Super-X Plex® Flow Cytometry Cytokine Assays Create your own custom cytokine panels! Select one or more assays from within each group/subcategory below. Or Multiplex ACROSS GROUPS with custom design by our lab. CUSTUMIZE YOUR MULTI-PLEX PANELS www.biocem.com.tr 16 Antigenix America Inc. Super-X Plex® Flow Cytometry Cytokine Assays Or choose from our ready-made multi-plex cytokine panels below: Human, Mouse, Rat, Non-Human Primate, or Canine Human Th1/Th2/Th17 Panel(7-Plex): Description/Target Human Th1/Th2/Th17(7-Plex Panel) Panel Description: Human IL-2 IL-4 IL-6 IL-10 IL-17A INF Gamma TNF ALPHA ANTIGEN STANDARTS: Human TH1/TH2/TH17 Panel CAT# HMX170 Super X Plex Kits Multi-Plex Panels SMX170 Pre-Mixed- (For customers who need EXTRA Set of standards) Human Inflammation Panel(7-Plex): Description/Target Human Inflammation(7-Plex Panel) Panel Description: Human IL-1 beta IL-1 RA IL-6 IL-8 IL-10 IL-12 p70 TNF ALPHA ANTIGEN STANDARTS: Human Inflammation Panel www.biocem.com.tr CAT# HMX270 SMX270 17 Cell Applications, Inc Primary Cells Cell Biology Products www.biocem.com.tr 18 Cell Applications, Inc Media Description Cat. No. Skeletal Muscle Cell Growth Medium 151-500 MesoEndo Cell Growth Medium 212-500 Endothelial Cell Growth Medium 211-500 Endothelial Cell Growth Medium Kit 211K-500 Smooth Muscle Cell Growth Medium 311-500 Smooth Muscle Cell Growth Medium Kit 311K-500 Synoviocyte Growth Medium 415-500 Human Mammary Epithelial Cell Growth Medium 815-500 Rat Brain Endothelial Growth Medium R819-500 Cells Description Cat. No. Short Human Epidermal Keratinocytes 102-05a HEK Human Epidermal Keratinocytes, neonatal 102-05n HEK Human Coronary Artery Endothelial Cells, adult 300-05a HCAEC Human Coronary Artery Smooth Muscle Cells 350-05a HCASMC Human Aortic Smooth Muscle Cells, adult 354-05a HAOSMC Human Chondrocytes Normal Tissue, adult 402-05a HC Human Fibroblasts-Like Synoviocytes, Normal Tissue, adult 408-05a HFLS Human Fibroblasts-Like Synoviocytes, Normal Tissue, adult 408RA-05a Human Fibroblasts-Like Synoviocytes, Normal Tissue, adult 602-05a HFDPC Human Mammary Epithelial Cells, adult 830-05a HMEpC Human Mammary Epithelial Cells, adult R357-25 RCm Rat Brain Microvascular Endothelial Cells, adult R840-05a RBMVEC www.biocem.com.tr HFLS-RA 19 TONBO Biosciences FCx-Pro™ Antibodies SPECIES SPECIFICITY FORMAT TESTS H CD3 FITC/PE/Purified 25-100 H CD3 APC/FITC/PE/PerCP-Cy5.5 25-100 H CD4 APC/FITC/PE/PerCP-Cy5.5 25-100-500 H CD19 APC/PE-Cy7/PE/PerCP-Cy5.5 25-100 H CD25 APC/PE/PE-Cy7/Purified 25-100 H CD45 APC/APC-Cy7/FITC/PE/PE-Cy7 25-100-500 H CD44 APC/APC-Cy7/FITC/PE/PE-Cy7 25-100 H IL-4 APC/In Vivo Ready/PE 25-100 H IL-17A APC/Alexa Fluor® 647 25-100 FCx-Product PRODUCT TESTS Ghost Dye™ Red 780 100-500 Ghost Dye™ Violet 450 100-500 Ghost Dye™ Violet 450 100-500 Ghost Dye™ Violet 510 100-500 Ghost Dye™ Violet 510 100-500 Propidium Iodide 100-500 CFSE 500ug 7-AAD 200-500 Cell Stimulation Cocktail (500X) 100 µL Flow Cytometry Perm Buffer (10X) 150 mL www.biocem.com.tr 20 Assay Kits & Reagents BioAssay Systems develops and markets innovative and high-throughput assay solutions to satisfy the ever increasing demands of the life sciences industry. BioAssay Systems offers assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. We focus on safe, non-radioactive formats such as absorbance, fluorescence and luminescence detection techniques. PRODUCTS CAT. NO. QuantiChrom™ Acetylcholinesterase Assay Kit DACE-100 QuantiChrom™ Calcium Assay Kit DICA-500 EnzyChrom™ Ethanol Assay Kit ECET-100 QuantiChrom™ Glucose Assay Kit DIGL-100 QuantiChrom™ Uric Acid Assay Kit DIUA-250 QuantiChrom™ Urea Assay Kit DIUR-500 EnzyChrom™ Triglyceride Assay Kit ETGA-200 QuantiChrom™ Zinc Assay Kit DIZN-250 EnzyChrom™ Superoxide Dismutase Assay Kit ESOD-100 EnzyChrom™ Nitric Oxide Synthase Assay Kit ENOS-100 EnzyChrom™ Catalase Assay Kit ECAT-100 QuantiChrom™ TBARS Assay Kit DTBA-100 EnzyFluo™ ERK Phosphorylation Assay Kit EERK-100 EnzyFluo™ NFKB Phosphorylation Assay Kit ENFKB-100 QuantiChrom™ Iron Assay Kit DIFE-250 EnzyChrom™ GSH/GSSG Assay Kit EGTT-100 EnzyChrom™ Glutathione Peroxidase Assay Kit EGPX-100 www.biocem.com.tr 21 G-Biosciences Educational Products Unlocking the Mysteries of Genetic Material APLICATIONS • Transformation of bacteria with a gene or DNA • Understand simple molecular biology and genetic manipulation techniques logy chno e • Role of bacteria as a production factory t o un Imm • Understand transformation in biotechnology Isolation & Characterization of Bacteria APLICATIONS • Cultivation of bacteria. • Aseptic handling techniques. • Bacteria isolation from test samples. • Characterize bacteria with Gram staining. • Applications of bacterial isolation gy biolo Micro Polymerase Chain Reaction (PCR) APLICATIONS • Teaches the principle of polymerase chain reaction (PCR) • Hands on PCR • Contains everything necessary for amplification of a gene of interest -­‐ cular Mole ogy Biol Enzyme Analysis APLICATIONS • Enzyme kinetic and regulation of enzyme activity. • Role of substrate concentration. • Effects of pH and temperature on the reaction rate. • Inhibition of enzyme reaction. d in an Prote omic Prote Immunoquantification Technique(ELISA) APLICATIONS • Teaches ELISA technique. • Learn to perform ELISA assay and screen unknown samples. • Simulates a real world ELISA test. Im logy chno ote mun Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides. www.biocem.com.tr 22 Santa Cruz Biotechnology, Inc. Primary Antibodies Santa Cruz Biotechnology now offers over 65.000 antibodies directed to a broad range of mammalian and non-mammalian protein targets. Bax An'body (N-­‐20): sc-­‐493 TLR2 An;body (S-­‐16): sc-­‐16237 p53 An'body (DO-­‐1): sc-­‐126 IRF-­‐1 Anibody (C-­‐20): sc-­‐497 β-­‐Ac'n An'body (C4): sc-­‐47778 α Tubulin An;body (YOL1/34): sc-­‐53030 CD45 An'body (H-­‐230): sc-­‐25590 PPARβ An;body (F-­‐7): sc-­‐74440 www.biocem.com.tr 23 Santa Cruz Biotechnology, Inc. Secondery Antibodies Western Blotting Conventional Secondary Antibodies Isotype-Specific Secondary Antibodies Cruz Marker Compatible Secondary Antibodies Western Blotting Control IgGs and IgG Conjugates Control Immunoglobulins and Conjugates IHC and Flow Cytometry Secondary Antibodies Conventional Secondary Antibodies Isotype-Specific Secondary Antibodies F(ab’)2 secondary antibodies Cruzfluor™ Secondary Antibody Conjugates IHC and Flow Cytometry Secondary Antibodies Normal Sera for Immunohistochemistry WB Positive Control Cell Lysates • HeLa Cell Lysate • A-431 Whole Cell Lysate • Jurkat Cell lysate Gene Silencer • Santa Cruz Biotechnology now offers siRNA and shRNA (plasmid and lentiviral) gene silencers targeted to all (100%) known human and mouse genes ImmunoCruz™ ImmunoCruz™ goat/mouse/rabbit LSAB Staining System • System includes 15 ml each of negative control IgG, peroxidase blocking solution, normal blocking serum, biotinylated secondary antibody and Avidin D-HRP reagent. • Each of the above reagents is pre-diluted and ready to use for immunohistochemical staining of tissue sections. • Also included are peroxidase substrate, DAB chromogen and DAB dilution buffer. • Number of slides: 150 ImmunoCruz™ rat/mouse/goat/rabbit ABC Staining System •System includes 1.0 ml normal blocking serum, 250 µg biotinylated se- condary antibody, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer. •Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate. •Number of slides: 200 www.biocem.com.tr 24 Santa Cruz Biotechnology, Inc. CRISPR/CAS9 Knockout Plasmids CRISPR/Cas9 SYSTEM The Clustered Regularly Interspaced Sorth Palindromic Repeats (CRISPR)/Cas9 system is an adaptive immune defense mechanism used by Archea and bacteria for the degradation of foreign genetic meterial. This mechanism has been repurposed for orher functions, including genomic engineering for mammalian systems, such as gene knockout. CRISPR/Cas9 PRODUCTS Each (CRISPR)/Cas9 Knockout (KO) Plasmid product consists of a pool o 3 plasmids designed to ensure identification and cleavage of a specific gene for maximum knockout efficiency. Each plasmid encodes a uniqe 20nt sequence derived from the GeCKO library. •Cas9/gRNA complex binds to proto-spacer adjacent to the (PAM) site and unwinds DNA. •gRNA binds to target locus in genomic DNA adjacent to the PAM site. •Cas9 cleves the 5’exons of the gene targeted by the three gRNA plasmids at three specific sites. •Target gene is distrupted. Santa Cruz Biotechnology offers HDR Plasmids specific to each CRISPR/Cas9 double strand break site. Santa Cruz offers CRISPR Double Nickase Plasmids ! Designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart. Santa Cruz offers CRISPR/dCas9 Activation Plasmids ! Designed to activate gene expression. Our CRISPR Plasmid product line now features: • CRISPR/Cas9 Knockout Plasmids for a very efficient bi-allelic or mono-allelic gene KO • HDR Plasmids for the homologous recombination i.e. repair of DNA DSB introduced by corresponding KO plasmids • Cre Vector sc-418923 to excise the genetic material inserted by HDR • CRISPR/dCas9 Activation Plasmids for transient activation/increase of gene transcription – 2 different products for 2 different target sequences ACT and ACT-2. • Double Nickase Plasmids for a very specific knockout of gene expression with minimal off- Target effects – 2 different products for 2 different target sequences offered NIC and NIC-2 www.biocem.com.tr 25 Santa Cruz Biotechnology, Inc. Chamicals Santa Cruz Biotechnology, Inc. now offers over 167,041 specialty biochemicals under our ChemCruz™ brand. Browse our product line below, which is organized in categories based on Specific Protein Interaction (5,890 chemicals), General Research Use (23,909 chemicals) or Chemical Class (158,333 chemicals). Individual chemicals may be found by searching for a CAS number, product name or formula. Biochemicals Laboratory Chemicals Dyes and Stains Fluorescent Probes Buffer Components Solvents Prepared Buffers HRP and AP Substrates www.biocem.com.tr 26 Santa Cruz Biotechnology, Inc. Buffers Dimethyl Sulfoxide (DMSO), cell culture reagent (CAS 67-68-5) Phosphate Buffered Saline: 1 L of 10X Tris Buffered Saline with Tween 20: 1 L of 10X Tris Buffered Saline: 1 L of 10X HEPES Buffer Solution (1M) (CAS 7365-45-9) Tris Buffered Saline with Tween 20: 1 L of 20X Phosphate Buffered Saline: 5 L of 20X Ponceau S solution (CAS 6226-79-5) Western Blot Stripping Buffer Tris Acetate-EDTA buffer, 50X BSA Blocking Buffer for ELISA HEPES sodium salt solution (1M) (CAS 75277-39-3) ChIP Wash Buffer Tris-EDTA buffer solution TRIS Glycine buffer solution Laemmli Sample Buffer (odor-free), 2X Laemmli Sample Buffer (odor-free), 2X Tris Base solution (CAS 77-86-1) www.biocem.com.tr 27 Santa Cruz Biotechnology, Inc. LABWARE UltraCruz™ Cell Scrapers, 40cm, sterile, 100/case: sc-213230 UltraCruz™ Cell Scrapers, 25cm, sterile, 100/case: sc-213229 UltraCruz™ Filter Flask, 150 ml, PES, 0.22 µm : sc-200309 UltraCruz™ Filter Flask, 150 ml, PES, 0.45 µm: sc-200310 UltraCruz™ Filter Flask, 250 ml, PES, 0.22 µm: sc-200253 UltraCruz™ Filter Flask, 250 ml, PES, 0.45 µm: sc-200254 UltraCruz™ Filter Flask, 500 ml, PES, 0.22 µm: sc-200255 UltraCruz™ Filter Flask, 500 ml, PES, 0.45 µm: sc-200256 UltraCruz™ Filter Flask, 1000 ml, PES, 0.22 μm: sc-359029 UltraCruz™ Filter Flask, 1000 ml, PES, 0.45 μm: sc-359028 UltraCruz™ Flask, Tissue Culture, 12.5 cm2, vent cap, :sc-200261 UltraCruz™ Flask, Tissue Culture, 25 cm2, vent cap, :sc-200262 UltraCruz™ Flask, Tissue Culture, 75 cm2, vent cap, :sc-200263 UltraCruz™ Flask, Tissue Culture, 182 cm2, vent cap, : sc-200264 UltraCruz™ Syringe Filter, PVDF, 0.10μm, 30mm, 30/case: sc-358810 UltraCruz™ Syringe Filter, PVDF, 0.22μm, 30mm, 30/case: sc-358812 UltraCruz™ Syringe Filter, PVDF, 0.45μm, 30mm, 30/case: sc-358814 Nylon Transfer Membrane, 0.45 µm, 30 cmx3 m roll : sc-201701 Nitrocellulose Transfer Membrane, 0.22µm,30cmx3mroll :sc-3718 PVDF Transfer Membrane, 0.45µm,30cmx3mroll :sc-3723 UltraCruz™ Tissue Culture Dish, 35 mm: sc-200284 UltraCruz™ Tissue Culture Dish, 60 mm: sc-351862 UltraCruz™ Tissue Culture Dish, 70 mm: sc-200285 UltraCruz™ Tissue Culture Dish, 100 mm: sc-200286 UltraCruz™ Petri Dish 35 mm : sc-351864 UltraCruz™ Petri Dish 60 mm : sc-351863 www.biocem.com.tr 28 Santa Cruz Biotechnology, Inc. LABWARE UltraCruz™ Tissue Culture Plates, 6 well, individually wrapped: sc-204443 UltraCruz™ Tissue Culture Plates, 12 well, individually wrapped: sc-204444 UltraCruz™ Tissue Culture Plates, 24 well, individually wrapped: sc-204445 UltraCruz™ Tissue Culture Plates, 48 well, individually wrapped: sc-204446 UltraCruz™ Tissue Culture Plates, 96 well, individually wrapped sc-204447 ExactaCruz™ Serological Pipet, 1ml, Individually Wrapped: sc-200275 ExactaCruz™ Serological Pipet, 2ml, Individually Wrapped: sc-200277 ExactaCruz™ Serological Pipet, 5ml, Individually Wrapped: sc-200279 ExactaCruz™ Serological Pipet, 10ml, Individually Wrapped: sc-200281 ExactaCruz™ Serological Pipet, 25ml, Individually Wrapped: sc-200283 ExactaCruz™ Serological Pipet, 50ml, Individually Wrapped: sc-213234 UltraCruz™ Centrifuge Tube, Conical, 15ml: sc-200250 UltraCruz™ Centrifuge Tube, Conical, 50ml: sc-200251 UltraCruz™ Single PCR Tube, 0,2 ml, pk: sc-205898 UltraCruz™ Microcentrifuge Tube, 0.5 ml, pk: sc-200269 UltraCruz™ Microcentrifuge Tube, 1.5 ml, pk: sc-200271 UltraCruz™ Microcentrifuge Tube, 2.0 ml, pk: sc-200273 UltraCruz™ Cryogenic Vials, Self Standing, PP, 1.2 ml : sc-204527 UltraCruz™ Cryogenic Vial, Self Standing, PP, 2 ml, : sc-204495 UltraCruz™ Cryogenic Vials, Self Standing, PP, 4 ml, : sc-204529 UltraCruz™ CryoBoxes : sc:200201 ExactaCruz™ Serological Pipet, 1ml, Individually Wrapped: sc-200275 ExactaCruz™ Serological Pipet, 2ml, Individually Wrapped: sc-200277 ExactaCruz™ Serological Pipet, 5ml, Individually Wrapped: sc-200279 ExactaCruz™ Serological Pipet, 10ml, Individually Wrapped: sc-200281 ExactaCruz™ Serological Pipet, 25ml, Individually Wrapped: sc-200283 ExactaCruz™ Serological Pipet, 50ml, Individually Wrapped: sc-213234 www.biocem.com.tr 29 Biocem Sağlık Ürünleri San. Tic. Ltd. Şti. Hizmetlerimiz Real-Time PCR Hizmeti Laboratuvarımızda qPCR uygulamalarında Applied Biosystems® marka 5 filtreye sahip ( FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red ve Cy5) 10-30 ul reaksiyon hacmiyle çalışmaya uygun 7500 Fast cihazı kullanılmaktadır. Laboratuarımızda aşağıdaki real-time uygulamaları konusunda hizmet verilmektedir: • • • • • Kalitatif analizler (İnternal pozitif kontrol kullanılarak var (plus) / yok (minus) analizleri) Absolute ve Relatif Gen Miktar Tayini Gen Ekspresyonu Analizi (ΔΔCT yöntemi) miRNA Gen Expresyon Analizi Genetik Varyasyon Analizi: SNP/CNV Genotipleme, İlaç Metabolizması Genotipleme, High-Resolution Melt Curve (HRM) PCR Hizmeti Laboratuarımızda bulunan Applied Biosystems® marka Veriti® 96-Well Thermal Cycler ve ProFlex™ PCR Sistemi (3x32-Well) cihazlarımız ve MiniBISPro jel görüntüleme cihazımız ile PCR, touch down PCR ve optimizasyon çalışmaları konusunda hizmet verilmektedir. Dizileme ve Fragman Analizi Hizmeti Dizileme ve Fragman Analizi konusunda Applied Biosystems®marka, 8 kapilerli 3500 cihazımız ile hızlı ve kaliteli sonuç alabilmekteyiz. Fragman ve dizi analizlerinde aşağıda verilen programlar ile güvenilir analiz hizmeti verilmektedir: • Sequencing Analysis Software • Sequencing – SeqScape® Software v2.7 • Fragment analysis – GeneMapper® Software v4.1 Ayrıca özel gen bölgeleri için dizi ve fragman primer dizaynı ve proje danışmanlık hizmeti verilmektedir. Fragman Analizi 1200 baz çiftine kadar hassas ve güvenilir fragment analizi yapılabilmektedir. Bu kapsamda aşağıdaki çalışmalar yapılmaktadır: • Microsatellite (STR) analizi • SNP Genotipleme • Fingerprinting • Relative Fluorescence Fragman analizi için florasan boya seçenekleri:6-FAM, VIC, NED, PED, LIZ (STANDART) Dizi Analizi Applied Biosystems®marka kullandığımız BigDye® Terminator v3.1 Cycle Sequencing Kit ile çift yönlü dizilemede 1000 baz çiftine kadar güvenilir ve hızlı dizi analizi hizmeti verilmektedir (QVs>20). Örneklerin hangi aşamadan itibaren işlem göreceğini (DNA izolasyonu-PCR-Cycle Sequencing-Dizileme ) bize bildirmeniz yeterlidir. Yeni Nesil Sekanslama Hizmeti Nükleotid bağlanması sırasında salınan hidrojen iyonlarının kullanarak bazların belirlenmesini sağlayan Ion Torrent™ sistemi ile istenilen çalışmaya uygun olan çipler (314, 316 ve 318) kullanılarak laboratuarımızda aşağıda yer alan hazır paneller veya kullanıcının isteğine özel genler ile oluşturulan dizayn paneller (Ion Community Panels) ile hizmet verilmektedir: Ion AmpliSeq Ready-to-use Panels; • Ion Ampliseq RNA Apoptosis Panel • • • • • Ion Ampliseq RNA Cancer Panel Ion Ampliseq Cancer Hotspot Panel v2 Ion Ampliseq Comprehensive Cancer Panel Ion Ampliseq Inherited Disease Panel Ion Ampliseq Cancer Panel Primer Pool Ion AmpliSeq Community Panels; • Ion Ampliseq CFTR Panel • Ion Ampliseq TP53 Panel • Ion Ampliseq BRCA1 ve BRCA2 Panel • Ion Ampliseq Colon ve Lung Cancer Panel • Dementia Research Gene Panel • Ion Ampliseq Human Identity Panel • Ion Ampliseq AML Panel ( gelecek! ) • Ion Ampliseq Cardio Panel ( gelecek!) Ayrıca yukarıdaki paneller dışında istenen gen bölgelerine ait panellerin custom olarak dizayn edilerek çalışılması imkanı da bulunmaktadır. www.biocem.com.tr 30 Biocem Sağlık Ürünleri San. Tic. Ltd. Şti. Notlar www.biocem.com.tr 31 Biocem Sağlık Ürünleri San. Tic. Ltd. Şti. Notlar www.biocem.com.tr 32
